Volume 11 CURRENT ISSUE
Effect of Garcinia Cambogia on Histopathological outcomes of Pancreatic Cells in Alloxan Induced Diabetic Albino Rat
1Dr Shumaila Shaikh, 2Dr Sadia Tabbassum, 3Dr. Asma Hingoro, 4Dr. Umair Azmat, 5Dr. Jawad Mumtaz Sodhar, 6Dr. Majid Ali Hingoro, 7Mehwish Jaweed, 8Prof. Dr Kashif Rasheed Shaikh, 9Dr Umair Ali Soomro, 10Prof Dr Muhammad Atif Ata
1Associate Professor, Department of Biochemistry, Khairpur Medical College, Khairpur, Sindh, Pakistan
2Associate Professor, Department of Biochemistry, Suleman Roshan Medical College, Tando Adam, Sindh, Pakistan
3Lecturer, Department of Pathology, Shaheed Mohtarma Benazir Bhutto Medical University, Larkana, Pakistan
4Demonstrator, Department of Pathology, Shahida Islam Medical and Dental College, Lodhran, Pakistan
5Associate Professor, Department of Pharmacology, Suleman Roshan Medical College, Tando Adam, Sindh, Pakistan
6Associate Professor, Department of Pharmacology, Mohi-udDin Islamic Medical College, Mirpur AJK, Pakistan
7Lecturer, Department of Management sciences, SZABIST University Hyderabad
8Department of Pharmacology, Suleman Roshan Medical College, Tando Adam, Sindh, Pakistan
9Associate Professor, Department of Pathology, Indus Medical College Tando Muhammad Khan, Sindh, Pakistan
10Department of Biochemistr, Suleman Roshan Medical College, Tando Adam, Sindh, Pakistan
Correspondence: Prof Dr Kashif Rasheed Shaikh, Department of Pharmacology, Suleman Roshan Medical College,Tando Adam, Sindh, Pakistan
Abstract
Background: Diabetes mellitus, is a prevalent metabolic condition categorised by continual hyperglycemia due to either impaired insulin secretion or resistance, has a high prevalence across Southeast Asia—ost notably in Pakistan. As such nations work fervently to control the disorder, scientists have begun exploring the potential of Garcinia cambogia extract to help regulate blood sugar levels. In this investigation, researchers concentrated on GcE’s impacts on pancreatic β-cell mass and islet morphology in diabetic rats highlighting the need for further research in alternative therapies for DM.
Methods: The investigation was designed as a pre-clinical experiment carried out over a twelve month duration at the Department of Pharmacology and Therapeutics, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi. A total of sixty young male albino Wistar rats were split into three sets which were induced to develop diabetes using Alloxan. The animals received differing doses of extracts derived from Garcinia cambogia termed GcE across an eight week timeframe. At the end of the defined experimental interval, the rats were anesthetized, sacrificed, and blood specimens were drawn to quantify fasting serum insulin levels under sedation.
Results: The mean weight significantly increased in Group C with a mean value of 304.95±35.15 gm as compared to group A and B with mean value of 292.05±43.15 gm and 294±50.16 gm respectively. Group C showed a significant increase in β-cell mass (355±55 units) compared to Groups A (280±35 units) and B (275±40 units), with improved islet shape (p<0.005).
Conclusion:The study concludes that Garcinia cambogia extract shows promise in enhancing pancreatic β-cell mass and islet morphologyin diabetes, suggesting potential therapeutic benefits.