Volume 11 Issue 6
Progressive Adaptation of a CpGV Isolate to Codling Moth Populations Resistant to CpGV-M
Benoît Graillot,Marie Berling,Christine Blachere-López,Myriam Siegwart,Samantha Besse andMiguel López-Ferber
1LGEI, Ecole des Mines d’Alès, Institut Mines-Telecom et Université de Montpellier Sud de France, 6, Avenue de Clavières, 30319 Alès, France
2Natural Plant Protection, Arysta LifeScience group, Avenue Léon Blum, 64000 Pau, France
3INRA, 6, Avenue de Clavières, 30319 Alès, France
4INRA, Unité PSH, Agroparc, 84914 Avignon Cedex 9, France
*Author to whom correspondence should be addressed.
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Present address: Créa, 215 Avenue de la Roche Parnale, ZI Motte Longue, 74130 Bonneville, France.
Abstract
The NPP-R1 isolate of CpGV is able to replicate on CpGV-M-resistant codling moths. However, its efficacy is not sufficient to provide acceptable levels of control in natural (orchard) conditions. A laboratory colony derived from resistant codling moths was established, which exhibited a homogeneous genetic background and a resistance level more than 7000 fold. By successive cycles of replication of NPP-R1 in this colony, we observed a progressive increase in efficacy. After 16 cycles (isolate 2016-r16), the efficacy of the virus isolate was equivalent to that of CpGV-M on susceptible insects. This isolate was able to control both CpGV-M-susceptible and CpGV-M-resistant insects with similar efficacy. No reduction in the levels of occlusion body production in susceptible larvae was observed for 2016-r16 compared to CpGV-M.
Keywords: Cydia pomonella granulovirus; coddling moth; resistance development; biological control