Volume 11 Issue 3
Surface Plasmon Resonator Using High Sensitive Resonance Telecommunication Wavelengths for DNA Sensors of Mycobacterium Tuberculosis with Thiol-Modified Probes
Shih-Hsiang Hsu,Shao-Chiang Hung,Yu-Kun Chen andZhi-Hao Jian
*Author to whom correspondence should be addressed.
Department of Electronic Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 10607, Taiwan
Abstract
Various analytes can be verified by surface plasmon resonance, thus continuous improvement of this sensing technology is crucial for better sensing selection and higher sensitivity. The SPR sensitivity on the wavelength modulation is enhanced with increasing wavelengths. The telecommunication wavelength range was then utilized to detect Mycobacterium tuberculosis (MTB) deoxyribonucleic acid (DNA) under two situations, without immobilization and with 5′-thiol end labeled IS6100 DNA probes, for SPR sensitivity comparison. The experimental data demonstrated that the SPR sensitivity increased more than 13 times with the wavelength modulation after immobilization. Since the operating wavelength accuracy of a tunable laser source can be controlled within 0.001 nm, the sensitivity and resolution on immobilized MTB DNA were determined as 1.04 nm/(μg/mL) and 0.9 ng/mL, respectively.Keywords: Mycobacterium tuberculosis; surface plasmon resonance; immobilization; wavelength modulation